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It will be seen, by reference to my sketch of the spot, Plate 20, that this conformation does actually exist there 293 with great definiteness: Turner has only enlarged and thrown it into more numerous alternations of light and shade.

The presence of cholesterol and phytosterol may be detected by dissolving a small portion of the unsaponifiable matter in acetic anhydride, and adding a drop of the solution to one drop of 50 per cent. sulphuric acid on a spot plate, when a characteristic blood red to violet coloration is produced.